Genetic resources and biotechnology: Consultant final report IICA/EMBRAPA-PROCENSUL II
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ISSN
0534-0591Date
08-1989Auteur
Instituto Interamericano de Cooperación para la Agricultura (IICA)
Maruniak, Jamer E
Empresa Brasileña de Investigación Agropecuaria (EMBRAPA)
Type
Consultoría
Metadata
Afficher la notice complèteDescription
24 páginas
Résumé
Research activities developed: 1) a rapid microextraction protocol for baculovirus DNA from either infected cell cultures or infected larvae was demonstrated. 2) Anticarsia baculovirus infected Anticarsia larvae brought from EMBRAPA Soja were tested for the effectiveness of the rapid microextraction protocol in purifying viral DNA. 3) Restriction enzyme analysis of microextracted viral DNA and gel electrophoresis were performed. 4) Reagents, cell culture supplies and titered Anticarsia baculovirus were brought to perform infected insect cell protein synthesis experiments. 5) Attempts were made to modify the rapid microextraction protocol to purify fungal mitochondrial DNA by using lysozyme to make fungal protoplasts before superating mitochondrial DNA from chromosomal DNA.
Delegation
Representación Brasil
Éditeur
IICA
URI (Lien permanent pour citer ou partager cet élément)
https://repositorio.iica.int/handle/11324/12509Collections
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