Genetic resources and biotechnology: Consultant final report IICA/EMBRAPA-PROCENSUL II

Abstract

Research activities developed: 1) a rapid microextraction protocol for baculovirus DNA from either infected cell cultures or infected larvae was demonstrated. 2) Anticarsia baculovirus infected Anticarsia larvae brought from EMBRAPA Soja were tested for the effectiveness of the rapid microextraction protocol in purifying viral DNA. 3) Restriction enzyme analysis of microextracted viral DNA and gel electrophoresis were performed. 4) Reagents, cell culture supplies and titered Anticarsia baculovirus were brought to perform infected insect cell protein synthesis experiments. 5) Attempts were made to modify the rapid microextraction protocol to purify fungal mitochondrial DNA by using lysozyme to make fungal protoplasts before superating mitochondrial DNA from chromosomal DNA.