Genetic resources and biotechnology: Consultant final report IICA/EMBRAPA-PROCENSUL II
View/ Open
ISSN
0534-0591Date
08-1989Author
Instituto Interamericano de Cooperación para la Agricultura (IICA)
Maruniak, Jamer E
Empresa Brasileña de Investigación Agropecuaria (EMBRAPA)
Type
Consultoría
Metadata
Show full item recordDescription
24 páginas
Abstract
Research activities developed: 1) a rapid microextraction protocol for baculovirus DNA from either infected cell cultures or infected larvae was demonstrated. 2) Anticarsia baculovirus infected Anticarsia larvae brought from EMBRAPA Soja were tested for the effectiveness of the rapid microextraction protocol in purifying viral DNA. 3) Restriction enzyme analysis of microextracted viral DNA and gel electrophoresis were performed. 4) Reagents, cell culture supplies and titered Anticarsia baculovirus were brought to perform infected insect cell protein synthesis experiments. 5) Attempts were made to modify the rapid microextraction protocol to purify fungal mitochondrial DNA by using lysozyme to make fungal protoplasts before superating mitochondrial DNA from chromosomal DNA.
Delegation
Representación Brasil
Publisher
IICA
URI (Permanet link to cite or share this item)
https://repositorio.iica.int/handle/11324/12509Collections
The following license files are associated with this item: